Reuters reported that the company had told it in an email: “We have now filed our appeal of the EC’s Android decision at the General Court of the EU."
Google had indicated at the time the fine was imposed that it would appeal to the second highest court in Europe.
In its appeal, the company cited arguments advanced by chief executive Sundar Pichai in a blog post when the fine was levied.
At the time the fine was imposed, EU competition commissioner Margrethe Vestager said: "Today, mobile Internet makes up more than half of global internet traffic. It has changed the lives of millions of Europeans.
"Our case is about three types of restrictions that Google has imposed on Android device manufacturers and network operators to ensure that traffic on Android devices goes to the Google search engine.
"In this way, Google has used Android as a vehicle to cement the dominance of its search engine. These practices have denied rivals the chance to innovate and compete on the merits.
"They have denied European consumers the benefits of effective competition in the important mobile sphere. This is illegal under EU anti-trust rules."
The July fine was the second levied by the EU on Google. In June last year, Google was fined €2.42 billion (US$2.7 billion) for allegedly abusing its search engine dominance to give illegal advantage to its own comparison shopping service. The company has appealed against this fine.
A third fine is said to be in the EU pipeline, this for alleged anti-business practices involving Google's AdSense advertising system.
The EU has also floated the idea of breaking up Google into a number of smaller units, with Vestager saying the political bloc harbours "grave suspicions" about the firm's dominance of the search market.
Brussels is not the only one to fine Google for anti-business practices. In February, the Competition Commission of India fined the company 135.86 crore rupees (about US$21.1 million) for "abusing its dominant position in online general Web search and Web search advertising services in India".
Google and other big multinational technology companies are also under pressure over alleged tax evasion, with the European Commission having unveiled a proposal to tax these companies at a rate of 3% as an interim measure.
Graphic: courtesy the European Union