On the network side, Telstra and Vocus are being joined by FirstPath and Western Australian Internet Association (WAIA).
"It is very exciting to see a new Equinix data centre in Melbourne," said WAIA president Pawel Mrugalski.
Cloud and IT service providers using ME1 include Bulletproof, Connectivity IT, Entrust ICT, Exigent and Servers Australia.
"In April 2014, Bulletproof opened its Melbourne office to support its regional operations," said Bulletproof marketing manager Lee-Sia May.
"Equinix's investment in ME1 provides Bulletproof with the ability to offer new and existing clients local network speeds in Victoria and bolster its hybrid solutions."
Content and enterprise customers are also being attracted to ME1. Tenants include CacheFly, Azzurri Communications and LiveBackup.
"From inception we realised that to be exceptional LiveBackup required the best storage, backup software, network, support and people, said GCOMM (LiveBackup's parent company) CEO Daniel Thompson.
"Although these individual components were equally important, ultimately LiveBackup also had to consider the bricks and mortar of where to house the service platform. Equinix ticked all the boxes and they continue to exceed our expectations."
Equinix Australia managing director Jeremy Deutsch said "With the tremendous momentum that we're seeing deploying in ME1, it is tangible proof that Equinix's interconnection solutions are key to customers' engaging with a dynamic ecosystem of business partners, networks and carriers.
"Enterprises are looking to be in closer proximity to their customers to reduce latency and improve application performance for their end-users. By deploying in Equinix, companies can do just that."